Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 1403-1412, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lipkin, L. E.
Right arrow Articles by Lemkin, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lipkin, L. E.
Right arrow Articles by Lemkin, P. F.

Clinical Chemistry, Vol 26, 1403-1412, Copyright © 1980 by American Association for Clinical Chemistry

Data-base techniques for multiple two-dimensional polyacrylamide gel electrophoresis analyses

LE Lipkin and PF Lemkin

Two-dimensional protein electrophoresis can benefit from a powerful set of computer-supported image processing and data structure/management procedures. Detection of quantitative differences is complicated by local inhomogeneities in the polyacrylamide base; biochemical changes and variations in temperature and preparative technique also make the between-gel density and x-y coordinate correspondences quite imprecise. The program presented here provides local alignment and computer- controlled variable "flicker" rates for multiple gels, with use of an interactive display system. Manual spot densitometry, referred to a National Bureau of Standards density wedge, can be complemented by a set of automatic densitometry routines for previously established lists of spots. The ability to establish a set of local landmarks, either by included standards or user identification, provides a basis for automatic n-way gel comparison for subsets or for the entire set of spots. Automatic segmentatin algorithms allow isolatin of spots and separation of touching and partially overlapping regions. Various analytical and statistical facilities are part of the user's access to the interactively developed data base. The data-structure and image- manipulation techniques developed here allow for user-directed and heuristic comparisons with online presentation of intermediate and final results.


The following articles in journals at HighWire Press have cited this article:


Home page
 Mol. Cell. ProteomicsHome page
S. Wolff, A. Otto, D. Albrecht, J. S. Zeng, K. Buttner, M. Gluckmann, M. Hecker, and D. Becher
Gel-free and Gel-based Proteomics in Bacillus subtilis: A Comparative Study
Mol. Cell. Proteomics, July 1, 2006; 5(7): 1183 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. F. Lemkin, G. C. Thornwall, K. D. Walton, and L. Hennighausen
The Microarray Explorer tool for data mining of cDNA microarrays: application for the mammary gland
Nucleic Acids Res., November 15, 2000; 28(22): 4452 - 4459.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. Davidson and A. Case
Rapid electronic autofluorography of labeled macromolecules on two-dimensional gels
Science, March 12, 1982; 215(4538): 1398 - 1400.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.