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Clinical Chemistry 26: 1419-1424, 1980;
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Clinical Chemistry, Vol 26, 1419-1424, Copyright © 1980 by American Association for Clinical Chemistry

Multiparameter appraoch to the "fingerprinting" of fluorescent Pseudomonads

DC Shelly, IM Warner and JM Quarles

A recent study has shown that an emission-excitation matrix could be used to provide a unique fingerprint for the selective identification and characterization of certain species of Pseudomonas. This paper describes the results of a systematic study of the variables that contribute to the uniqueness of the fingerprint. Additional insight concerning the nature of the fluorescent pigments of these species is gained by analysis with a high-performance liquid chromatograph/video fluorometer combination. While providing information about the analytical variables contributing to the analysis, this combination also added the variable of chromatographic retention time for unambiguous fingerprinting.




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