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Clinical Chemistry 26: 1504-1508, 1980;
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Clinical Chemistry, Vol 26, 1504-1508, Copyright © 1980 by American Association for Clinical Chemistry

Serum protein profiles by "high-performance" liquid chromatography with detection at multiple wavelengths

TD Schlabach and SR Abbott

We have separated serum proteins in only 15 min by high-performance liquid chromatography. The identification of several peaks in the chromatographic profile was greatly aided by the use of multiple- wavelength detection. We have found a good correlation between retention times and electrophoretic mobilities. Serum samples with increased gamma- or beta-globulins, as determined by electrophoresis, resulted in chromatographic profiles with strongly increased peaks in the appropriate regions. This chromatographic method revealed both relative and absolute differences in individual protein concentrations among several serum samples.




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Copyright © 1980 by the American Association for Clinical Chemistry.