Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 1540-1543, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okabe, H.
Right arrow Articles by Noma, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okabe, H.
Right arrow Articles by Noma, A.

Clinical Chemistry, Vol 26, 1540-1543, Copyright © 1980 by American Association for Clinical Chemistry

Enzymic determination of free fatty acids in serum

H Okabe, Y Uji, K Nagashima and A Noma

We describe the enzymic determination of free fatty acids in serum with use of acyl-CoA synthetase (EC l.2.1.3), acyl-CoA oxidase (no EC no. assigned) and peroxidase (EC 1.11.1.7). The free fatty acids are activated by acyl-CoA synthetase in the presence of ATP and coenzyme A. The acyl-CoA formed is oxidized by acyl-CoA oxidase to enoyl-CoA with simultaneous production of hydrogen peroxide, which is oxidatively coupled with 4-amino-antipyrine and 2,4-dibromphenol in the presence of peroxidase to yield a product that absorbs maximally at 505 nm. Standard curves prepared with various kinds of fatty acids are linear to at least 2.0 mmol/L and are practically congruent. Ascorbic acid or bilirubin interferes slightly. Results by the present method correlate well with those by the colorimetric method involving 2-(2-thiozolylazo)- p-cresol (r = 0.98). Replicate analyses of standard solution and of two kinds of control sera demonstrated the following between-assay precision: mean absorbance 0.224 (SD 0.002), CV 0.85%; mean concentration, 326 (SD 7.3) and 1076 (SD 22.7) mumol/L, CV 2.24 and 2.11%, respectively.


The following articles in journals at HighWire Press have cited this article:


Home page
 Hum ReprodHome page
P. Villa, L. Soranna, A. Mancini, L. De Marinis, D. Valle, S. Mancuso, and A. Lanzone
Effect of feeding on growth hormone response to growth hormone-releasing hormone in polycystic ovarian syndrome: relation with body weight and hyperinsulinism
Hum. Reprod., March 1, 2001; 16(3): 430 - 434.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
N. Paquot, M. J. Castillo, P. J. Lefèbvre, and A. J. Scheen
No Increased Insulin Sensitivity after a Single Intravenous Administration of a Recombinant Human Tumor Necrosis Factor Receptor: Fc Fusion Protein in Obese Insulin-Resistant Patients
J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 1316 - 1319.
[Abstract] [Full Text]

Home page
 Cardiovasc ResHome page
K. F. Kofoed, H. Schoder, R. J. Knight, and D. B. Buxton
Glucose metabolism in reperfused myocardium measured by [2-18F] 2-fluorodeoxyglucose and PET
Cardiovasc Res, January 14, 2000; 45(2): 321 - 329.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
B. Guerci, L. Meyer, A. Sallé, A. Charrié, B. Dousset, O. Ziegler, and P. Drouin
Comparison of Metabolic Deterioration between Insulin Analog and Regular Insulin after a 5-Hour Interruption of a Continuous Subcutaneous Insulin Infusion in Type 1 Diabetic Patients
J. Clin. Endocrinol. Metab., August 1, 1999; 84(8): 2673 - 2678.
[Abstract] [Full Text]

Home page
 J. Nutr.Home page
J.-F. Gabarrou, P.-A. Géraert, M. Picard, and A. Bordas
Diet-Induced Thermogenesis in Cockerels Is Modulated by Genetic Selection for High or Low Residual Feed Intake
J. Nutr., December 1, 1997; 127(12): 2371 - 2376.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Leal-Cerro, L. M. Jimenez, R. Astorga, I. Fernandez-Lopez, C. Dieguez, and F. F. Casanueva
Acute Pharmacological Reduction of Plasma Free Fatty Acids Enhances the Growth Hormone (GH)-Releasing Hormone-Mediated GH Secretion in Patients with Cushing's Syndrome
J. Clin. Endocrinol. Metab., September 1, 1997; 82(9): 3165 - 3168.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
P.D. Bartels and H. Lund-Jacobsen
Blood Lactate and Ketone Body Concentrations in Salicylate Intoxication
Human and Experimental Toxicology, December 1, 1986; 5(6): 363 - 366.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.