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Clinical Chemistry 26: 1620-1621, 1980;
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Clinical Chemistry, Vol 26, 1620-1621, Copyright © 1980 by American Association for Clinical Chemistry

Detection of alkaline phosphatase/immunoglobulin complexes

JE Buttery, CR Milner, P Nenadovic and PR Pannall

We report another patient with a circulating alkaline phosphatase/immunoglobulin complex in his blood, and describe a simple method of demonstrating such complexes. On electrophoresis on cellulose acetate, the complex was relatively slow moving and there was no activity in the normal bone/liver isoenzyme region. When the serum was treated with trypsin, the slow band disappeared and the normal pattern was restored.




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