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Clinical Chemistry 26: 1666-1671, 1980;
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Clinical Chemistry, Vol 26, 1666-1671, Copyright © 1980 by American Association for Clinical Chemistry

C-terminal parathyrin (parathyroid hormone) radioimmunoassay in serum with commercially available reagents

M Simon and J Cuan

We describe a sequential double-antibody assay for measuring C-terminal parathyrin in serum with commercially available reagents. Intact bovine hormone, used as a working standard, is iodinated by conventional Chloramine T procedures. The antibody affinity is characteristic of high affinity binding (1.4 to 1.6 x 10(10) L/mol of intact parathyrin). The antibody also cross reacts with a C-terminal parathyrin fragment (amino acids 53-84) but not with a synthetic N-terminal parathyrin fragment (amino acids 1-34). The assay thus also measures both a C- terminal fragment of parathyrin and the intact hormone. The detection limit (250 ng/L; 500 int. units/L) is below the reference interval for healthy adults (430-1860 ng/L; 860-3720 int. units/L). Several commonly recognized problems with iodinated parathyrin are eliminated and accuracy and precision of the procedures for standard preparation and calibration are improved. Overall CV (between-run imprecision) is 10- 17%. Analytical recovery is 80-90%, and C-terminal parathyrin measured in fresh sera and in sera stored for seven days at 30 degrees C is equivalent.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
C. Bieglmayer, G. Prager, and B. Niederle
Kinetic Analyses of Parathyroid Hormone Clearance as Measured by Three Rapid Immunoassays during Parathyroidectomy
Clin. Chem., October 1, 2002; 48(10): 1731 - 1738.
[Abstract] [Full Text] [PDF]


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