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Clinical Chemistry 26: 1694-1700, 1980;
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Clinical Chemistry, Vol 26, 1694-1700, Copyright © 1980 by American Association for Clinical Chemistry

An immunochemical procedure for determination of mitochondrial aspartate aminotransferase in human serum

R Rej

An immunochemical procedure is described for quantitation of mitochondrial aspartate aminotransferase (m-AspAT; EC 2.6.1.1) activity in human serum specimens. Antibodies directed against purified soluble aspartate aminotransferase (s-AspAT) from human erythrocytes were produced in rabbits and partly purified. Antibody sufficient for analyses of > 6000 specimens could be obtained from 15 mL of rabbit antiserum; contaminant AspAT activity of the antibody preparation was < 0.4 U/L. Addition of antibody directly to purified AspAT isoenzymes resulted in inhibition of s-AspAT but had no measurable effect upon m- AspAT. Antibody is incubated with serum in the presence of polyethylene glycol for 60 min at room temperature, then 60 min at 4 degrees C, and centrifuged (7000 x g, 4 degrees C, 15 min). No detectable s-AspAT activity remains in the supernatant fluid; thus m-AspAT activity can be measured directly. Precision, both within-day and day-to-day, was < 1 U/L, or 3.0% of residual m-AspAT activity. The method completely removed 1200 U of purified s-AspAT activity per liter; addition of s- AspAT to serum in increasing concentrations of about 500 U/L had no effect upon the measurement of residual m-AspAT activity. Results of the procedure described showed excellent correlation with those by an alternative procedure involving antibodies directed against m-AspAT. Addition of both anti-s- and anti-m-AspAT antibodies resulted in complete removal of serum AspAT activity. Univalent Fab fragments prepared anti-s-AspAT antibodies were capable of directly inhibiting s- AspAT activity without precipitation. Although a homogeneous immunoinhibition assay was possible, the greater precision of the precipitation assay made it preferable.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
S. Y. Jeong, K. J. Kim, D. J. Kim, S. W. Oh, and E. Y. Choi
Sandwich ELISA for Measurement of Cytosolic Aspartate Aminotransferase in Sera from Patients with Liver Diseases
Clin. Chem., May 1, 2003; 49(5): 826 - 829.
[Full Text] [PDF]

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 Br J AnaesthHome page
J. R. Darling, P. C. Sharpe, E. K. Stiby, J. A. McAteer, G. P. R. Archbold, and K. R. Milligan
Serum mitochondrial aspartate transaminase activity after isoflurane or halothane anaesthesia
Br. J. Anaesth., August 1, 2000; 85(2): 195 - 198.
[Abstract] [Full Text] [PDF]


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