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Clinical Chemistry 26: 190-193, 1980;
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Clinical Chemistry, Vol 26, 190-193, Copyright © 1980 by American Association for Clinical Chemistry

Semi-automated fluorometry of total estrogens in plasma during late pregnancy

C Goutte-Coussieu, D Sanchez, G Adessi and MF Jayle

We describe a mechanized method for determining total estrogens in plasma during the last trimester of pregnancy. This method involves rapid enzymic hydrolysis, automatic extraction (in a tube) with cyclohexane/ethyl acetate, purification of the extracted material by anion-exchange chromatography on a disposable "mini-column" of Dowex AG1 X 2 (acetate form), and continuous-flow fluorometric quantification. Conditions of hydrolysis and extraction were studied. The mean within-day imprecision (CV) was 7% and between-day imprecision was 8.1%. Sensitivity was 75 nmol/L of plasma. Results are obtained in 4 h. The technique is quite suitable for routine determinations: 90 assays can be done by a team of three technicians in one working day.




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Copyright © 1980 by the American Association for Clinical Chemistry.