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Clinical Chemistry 26: 403-405, 1980;
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Clinical Chemistry, Vol 26, 403-405, Copyright © 1980 by American Association for Clinical Chemistry

Amniotic fluid phospholipids measured by continuous-development thin- layer chromatography

MD Kolins, E Epstein, WH Civin and S Weiner

We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.




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