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Clinical Chemistry 26: 406-408, 1980;
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Clinical Chemistry, Vol 26, 406-408, Copyright © 1980 by American Association for Clinical Chemistry

Radioimmunoassay of thyroxine in 10 microliters of serum, with use of aggregated antithyroxine antibodies

S Collins, M Brooks and EW Bermes

We describe a sensitive radioimmunoassay for rapidly determining the concentration of thyroxine in 10 microL of human serum. Aggregated antithyroxine antibodies are used to separate bound and free hormone. This speeds the assay and economizes on reagents without loss of sensitivity, specificity, or precision. Results for normal subjects and patients with thyroid disease agree well with those obtained by other, well-established techniques.




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Copyright © 1980 by the American Association for Clinical Chemistry.