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Clinical Chemistry 27: 146-148, 1981;
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Clinical Chemistry, Vol 27, 146-148, Copyright © 1981 by American Association for Clinical Chemistry

Liquid-chromatographic quantification of plasma phenylalanine, tyrosine, and tryptophan

LM Neckers, LE Delisi and RJ Wyatt

Phenylalanine, tyrosine, and tryptophan are isolated and quantified by "high-pressure" liquid chromatography, with fluorescence detection. An isocratic mobile phase and reversed-phase column are used to provide rapid and reproducible measurement of these amino acids in as little as 1 to 2 microL of human plasma.




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Copyright © 1981 by the American Association for Clinical Chemistry.