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Clinical Chemistry 27: 2041-2043, 1981;
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Clinical Chemistry, Vol 27, 2041-2043, Copyright © 1981 by American Association for Clinical Chemistry

Liquid-chromatographic monitoring of 5-methyltetrahydrofolate in plasma

P Giulidori, M Galli-Kienle and G Stramentinoli

The liquid-chromatographic measurement of 5-methyltetrahydrofolate in biological fluids is described. The sensitivity of the spectrophotofluorometric detector used allows direct evaluation of basal concentrations of the compound in plasma. Because it is resolved from the other common folates and from methotrexate, the procedure is suitable for monitoring it in plasma of patients receiving high-dose therapy with methotrexate.


The following articles in journals at HighWire Press have cited this article:


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 J. Biol. Chem.Home page
E.-J. Yeo, W. T. Briggs, and C. Wagner
Inhibition of Glycine N-Methyltransferase by 5-Methyltetrahydrofolate Pentaglutamate
J. Biol. Chem., December 31, 1999; 274(53): 37559 - 37564.
[Abstract] [Full Text] [PDF]


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Copyright © 1981 by the American Association for Clinical Chemistry.