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Clinical Chemistry 27: 2060-2063, 1981;
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Clinical Chemistry, Vol 27, 2060-2063, Copyright © 1981 by American Association for Clinical Chemistry

Urinary free catecholamines determined by liquid chromatography-- fluorometry

GM Anderson, JG Young, PI Jatlow and DJ Cohen

Free norepinephrine, epinephrine, and dopamine are determined in urine after purification on a small ion-exchange column and concentration on alumina. The catecholamines are separated by reversed-phase ion-pair chromatography and are quantitated by measuring their native fluorescence (lambda ex 285 nm, lambda em 305 nm). The internal standard is dihydroxybenzylamine. Within-day CVs for the compounds ranged from 1.6 to 5.6%; between-day CVs were 6.6 to 9.8%.




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