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Clinical Chemistry 27: 901-905, 1981;
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Clinical Chemistry, Vol 27, 901-905, Copyright © 1981 by American Association for Clinical Chemistry

Enzyme immunoassay for specific determination of the synthetic estrogen, ethynyl estradiol, in plasma

A Turkes, J Dyas, GF Read and D Riad-Fahmy

In this enzyme immunoassay for ethynyl estradiol, a conjugate of the 3- (O-carboxymethyl)ether with horseradish peroxidase is used as the label and a conjugate of the 6-(O-carboxymethyl)oxime with bovine serum albumin as the immunogen. A solid-phase antibody procedure is used in separating antibody-bound and free steroid. The lower limit of sensitivity of the assay is 2 pg per assay tube. Interference by synthetic progestagens was minimized by extracting the sample with an anti-estrogen serum before assay. Results obtained with a comparison radioimmunoassay and this procedure agreed well (r greater than 0.98).




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Copyright © 1981 by the American Association for Clinical Chemistry.