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Clinical Chemistry 27: 910-913, 1981;
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Clinical Chemistry, Vol 27, 910-913, Copyright © 1981 by American Association for Clinical Chemistry

Steroid radioimmunoassay--effect of shortened incubation time on specificity

RF Vining, P Compton and R McGinley

Use of a briefer incubation interval in several steroid radioimmunoassays markedly increases cross reactions with some closely related steroids. The dissociation rates of the various steroid/antibody complexes play a critical role in determining the specificity of the antiserum, and the maximum specificity of an antiserum will be exploited only if it is incubated to equilibrium. The commonly used method for estimating the time required to reach equilibrium--i.e., the time required for the %(B0/T) value to "plateau"- -grossly underestimates the true interval required, and we suggest that the minimum incubation period for steroid radioimmunoassay should be based on a knowledge of both the dissociation and the association rate.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Valdes Jr. and S. A. Jortani
Unexpected Suppression of Immunoassay Results by Cross-Reactivity: Now a Demonstrated Cause for Concern
Clin. Chem., March 1, 2002; 48(3): 405 - 406.
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