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Clinical Chemistry 27: 930-932, 1981;
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Clinical Chemistry, Vol 27, 930-932, Copyright © 1981 by American Association for Clinical Chemistry

Effect of centrifugation on fluorescence polarization of amniotic fluid

NV Simon, RC Elser, JS Levisky and DT Polk

Fluorescence polarization was measured on amniotic fluids of different gestational ages before and after centrifugation at relative centrifugal forces of 34 to 4955 x g. Centrifugation significantly increased the fluorescence polarization values over those of uncentrifuged amniotic fluids. The greatest increase was observed in the most "mature" fluids centrifuged at 34 to 230 x g. Almost without exception, use of centrifugal forces greater than 1239 x g did not further increase fluorescence polarization values.




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Copyright © 1981 by the American Association for Clinical Chemistry.