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Clinical Chemistry 27: 1335-1340, 1981;
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Clinical Chemistry, Vol 27, 1335-1340, Copyright © 1981 by American Association for Clinical Chemistry

Proteins of human semen. I. Two-dimensional mapping of human seminal fluid

JJ Edwards, SL Tollaksen and NG Anderson

The proteins in human seminal plasma were mapped by high-resolution two- dimensional electrophoresis (ISO-DALT and BASO-DALT systems). When analyzed under dissociating conditions, samples from normal fertile males revealed a pattern of over 200 proteins, ranging in mass from 10 000 to 100 000 daltons. Comparison of the mapped proteins from these males and those who had undergone vasectomy allowed us to identify one series of glycoproteins as missing from the semen from vasectomized individuals. Glycoproteins isolated by affinity chromatography with use of concanavalin A were also mapped. Some of the protein spots were identified either by co-electrophoresis with purified proteins or by the electrophoretic transfer of proteins to nitrocellulose sheets and subsequent detection by immunological procedures. The proteins identified include a number of serum proteins as well as prostatic acid phosphatase and creatine kinase. Proteolytic events shown to occur during the liquefaction of semen that occurs early after collection indicate the importance of carefully controlled collection and preparation methods for clinical evaluation of seminal plasma. Ethylenediaminetetraacetic acid and phenylmethylsulfonyl fluoride inhibit this proteolysis.


The following articles in journals at HighWire Press have cited this article:


Home page
 J AndrolHome page
K. Yamakawa, K. Yoshida, H. Nishikawa, T. Kato, and T. Iwamoto
Comparative Analysis of Interindividual Variations in the Seminal Plasma Proteome of Fertile Men With Identification of Potential Markers for Azoospermia in Infertile Patients
J Androl, November 1, 2007; 28(6): 858 - 865.
[Abstract] [Full Text] [PDF]

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 FASEB J.Home page
G. UNTERGASSER, H. RUMPOLD, E. PLAS, S. MADERSBACHER, and P. BERGER
A low-molecular-weight fraction of human seminal plasma activates adenylyl cyclase and induces caspase 3-independent apoptosis in prostatic epithelial cells by decreasing mitochondrial potential and Bcl-2/Bax ratio
FASEB J, March 1, 2001; 15(3): 673 - 683.
[Abstract] [Full Text] [PDF]


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Copyright © 1981 by the American Association for Clinical Chemistry.