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Clinical Chemistry 27: 1388-1391, 1981;
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Clinical Chemistry, Vol 27, 1388-1391, Copyright © 1981 by American Association for Clinical Chemistry

Automated two-site immunoradiometric assay of human alpha-fetoprotein in maternal serum

HA Kemp, JS Simpson and JS Woodhead

Immunoassays of alpha 1-fetoprotein in maternal serum are now in widespread use as a means of antenatal detection of neural tube defects. For population screening to be effective the initial test must be capable of promptly producing reliable results. We describe an automated two-site immunoradiometric assay based on 125I-labeled antibodies and cellulose-coupled antibody, with which up to 100 patients' samples can be assayed in 3 h. The assay is precise, and results obtained agree closely with those produced by conventional radioimmunoassay procedures.




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Copyright © 1981 by the American Association for Clinical Chemistry.