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Clinical Chemistry 28: 19-24, 1982;
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Clinical Chemistry, Vol 28, 19-24, Copyright © 1982 by American Association for Clinical Chemistry

Macro creatine kinase: determination and differentiation of two types by their activation energies

W Stein, J Bohner, R Steinhart and M Eggstein

Determination of the MB isoenzyme of creatine kinase in patients with acute myocardial infarction may be disturbed by the presence of macro creatine kinase. The relative molecular mass of this form of creatine kinase in human serum is at least threefold that of the ordinary enzyme, and it is more thermostable. Here we describe our method for determination of macro creatine kinases and an easy-to-perform test for differentiating two forms of macro creatine kinase, based on their distinct activation energies. The activation energies of serum enzymes are mostly in the range of 40-65 kJ/mol of substrate. Unlike normal cytoplasmatic creatine kinases and IgG-linked CK-BB (macro creatine kinase type 1) a second form of macro creatine kinase (macro creatine kinase type 2) shows activation energies greater than 80 kJ/mol of substrate. The exact composition of macro creatine kinase type 2 is still unknown, but there is good reason to believe that it is of mitochondrial origin.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
A. H. B. Wu, Y.-J. Feng, J. Nadelman, M. Acampora, and P. N. Fiedler
Ectopic Production of Creatine Kinase MB: Updated Evaluation by Mass Assays
Clin. Chem., October 1, 1997; 43(10): 2006 - 2007.
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Copyright © 1982 by the American Association for Clinical Chemistry.