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Clinical Chemistry 28: 5-8, 1982;
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Clinical Chemistry, Vol 28, 5-8, Copyright © 1982 by American Association for Clinical Chemistry

Comparison of two isotope dilution/mass spectrometric methods for determination of total serum cholesterol

R Schaffer, LT Sniegoski, MJ Welch, VE White, A Cohen, HS Hertz, J Mandel, RC Paule, L Svensson, I Bjorkhem and R Blomstrand

Isotope dilution/mass spectrometric methods for total serum cholesterol, developed separately at the Karolinska Institutet (KI) and the National Bureau of Standards (NBS), were compared by applying them to a common set of serum pools. A search for the cause of a systematic difference of a few percent in results from the two methods revealed that the KI cholesterol standard contained lathosterol, which interfered with the calibration of the method. With NBS Standard Reference Material cholesterol used for new analyses at the KI, the average difference in mean values dropped to 0.2%. The NBS results are more precise. This is attributed to the protocols NBS used for sample preparation and mass spectrometry. However, these protocols make the NBS method more complex and time-consuming. A recent critical article on the use of this technique for total cholesterol is also examined.


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Copyright © 1982 by the American Association for Clinical Chemistry.