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Clinical Chemistry 28: 83-86, 1982;
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Clinical Chemistry, Vol 28, 83-86, Copyright © 1982 by American Association for Clinical Chemistry

Morphine glucuronide hydrolysis: superiority of beta-glucuronidase from Patella vulgata

J Combie, JW Blake, TE Nugent and T Tobin

beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized. A 3-h incubation at 65 degrees C with 5000 U of beta-glucuronidase from P. vulgata per milliliter of urine resulted in complete hydrolysis of all morphine glucuronide in the urine samples. Not only was the enzyme preparation from P. vulgata the most cost-effective beta-glucuronidase source studied, but also its thermal stability is such that it can be used at a temperature high enough to substantially shorten the incubation interval. Preliminary work on other drugs that form glucuronide conjugates indicates that this same procedure is similarly superior for use in their hydrolysis.


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S. W. Toennes and H. H. Maurer
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Intestinal Absorption and Stability of Morphine 6-Glucuronide in Different Physiological Compartments of the Rat
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