Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 28: 2088-2094, 1982;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klenk, D. C.
Right arrow Articles by Goldstein, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klenk, D. C.
Right arrow Articles by Goldstein, D. E.

Clinical Chemistry, Vol 28, 2088-2094, Copyright © 1982 by American Association for Clinical Chemistry

Determination of glycosylated hemoglobin by affinity chromatography: comparison with colorimetric and ion-exchange methods, and effects of common interferences

DC Klenk, GT Hermanson, RI Krohn, EK Fujimoto, AK Mallia, PK Smith, JD England, HM Wiedmeyer, RR Little and DE Goldstein

An affinity-chromatographic method for determination of glycosylated hemoglobin (Anal. Lett. 14: 649-661, 1981) is compared with the thiobarbituric acid colorimetric (I) (Clin. Chem. 27: 669-672, 1981) and the ion-exchange liquid-chromatographic (II) (Diabetes 29: 623-628, 1980) methods. A correlation of 0.98 was obtained for the affinity method vs II and 0.97 for affinity vs I (n = 51). The within-run CV was 1.9% for specimens from non-diabetic individuals and 1.0% for those from diabetics. The respective between-run CVs were 3.4% and 2.4%. Failure to remove "labile" glucose adducts by 5-h incubation of erythrocytes in isotonic saline (37 degrees C) contributed an average error of 13.1% for II, 5.4% for I, and 1.6% for the affinity method. Affinity chromatography gave a decrease of 0.1-0.2% glycosylated hemoglobin for each 1.0 degree C temperature increase between 18 and 27 degrees C. Varying the pH of the wash buffer used in the affinity procedure from 7.75 to 8.25 (pH 8.0 optimum) produced at net change of 0.5% in glycosylated hemoglobin with one diabetic specimen. Using the affinity method, we determined the reference interval for glycosylated hemoglobin in 124 apparently healthy individuals to be 5.3 to 7.5% (mean 6.36%, SD 0.55%). Rechromatography by II and isoelectric focusing analysis of the fractions obtained by the affinity separation revealed a substantial population of glycosylated hemoglobins not measured by II. The affinity method offers a rapid, simple, precise, and accurate alternative to methods currently in use and gives substantial freedom from many common interferences.


The following articles in journals at HighWire Press have cited this article:


Home page
 Arch OphthalmolHome page
R. Klein, S. E. Moss, S. M. Meuer, and B. E. K. Klein
The 15-Year Cumulative Incidence of Retinal Vein Occlusion: The Beaver Dam Eye Study
Arch Ophthalmol, April 1, 2008; 126(4): 513 - 518.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
S. Miura, S. Ferri, W. Tsugawa, S. Kim, and K. Sode
Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis
Protein Eng. Des. Sel., April 1, 2008; 21(4): 233 - 239.
[Abstract] [Full Text] [PDF]

Home page
 Psychosom. Med.Home page
V. K. Tsenkova, G. D. Love, B. H. Singer, and C. D. Ryff
Socioeconomic Status and Psychological Well-Being Predict Cross-Time Change in Glycosylated Hemoglobin in Older Women Without Diabetes
Psychosom Med, October 1, 2007; 69(8): 777 - 784.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. E. Lee, B. E. K. Klein, R. Klein, and M. D. Knudtson
Familial Aggregation of Retinal Vessel Caliber in the Beaver Dam Eye Study
Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 3929 - 3933.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. R. Teixeira, K. A. Tappenden, L. Carson, R. Jones, M. Prabhudesai, W. P. Marshall, and J. W. Erdman Jr.
Isolated Soy Protein Consumption Reduces Urinary Albumin Excretion and Improves the Serum Lipid Profile in Men with Type 2 Diabetes Mellitus and Nephropathy
J. Nutr., August 1, 2004; 134(8): 1874 - 1880.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
N. Marx, D. Walcher, C. Raichle, M. Aleksic, H. Bach, M. Grub, V. Hombach, P. Libby, A. Zieske, S. Homma, et al.
C-Peptide Colocalizes with Macrophages in Early Arteriosclerotic Lesions of Diabetic Subjects and Induces Monocyte Chemotaxis In Vitro
Arterioscler. Thromb. Vasc. Biol., March 1, 2004; 24(3): 540 - 545.
[Abstract] [Full Text]

Home page
 IOVSHome page
T. Y. Wong, R. Klein, B. E. K. Klein, S. M. Meuer, and L. D. Hubbard
Retinal Vessel Diameters and Their Associations with Age and Blood Pressure
Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4644 - 4650.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
R. Klein, B. E. K. Klein, S. E. Moss, and S. M. Meuer
Retinal Emboli and Cardiovascular Disease: The Beaver Dam Eye Study
Arch Ophthalmol, October 1, 2003; 121(10): 1446 - 1451.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
B. E.K. Klein, R. Klein, and K. E. Lee
Components of the Metabolic Syndrome and Risk of Cardiovascular Disease and Diabetes in Beaver Dam
Diabetes Care, October 1, 2002; 25(10): 1790 - 1794.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. E. Lee, B. E. K. Klein, R. Klein, and T. Y. Wong
Changes in Refraction over 10 Years in an Adult Population: The Beaver Dam Eye Study
Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2566 - 2571.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
R. S. Surwit, M. A.L. van Tilburg, N. Zucker, C. C. McCaskill, P. Parekh, M. N. Feinglos, C. L. Edwards, P. Williams, and J. D. Lane
Stress Management Improves Long-Term Glycemic Control in Type 2 Diabetes
Diabetes Care, January 1, 2002; 25(1): 30 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H. C. McGill Jr, C. A. McMahan, E. E. Herderick, R. E. Tracy, G. T. Malcom, A. W. Zieske, and J. P. Strong
Effects of Coronary Heart Disease Risk Factors on Atherosclerosis of Selected Regions of the Aorta and Right Coronary Artery
Arterioscler. Thromb. Vasc. Biol., March 1, 2000; 20(3): 836 - 845.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
J. W. Anderson, M. S. Gowri, J. Turner, L. Nichols, V. A. Diwadkar, C. K. Chow, and P. R. Oeltgen
Antioxidant Supplementation Effects on Low-Density Lipoprotein Oxidation for Individuals with Type 2 Diabetes Mellitus
J. Am. Coll. Nutr., October 1, 1999; 18(5): 451 - 461.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
R. Klein, B. E. K. Klein, S. C. Jensen, S. E. Moss, and S. M. Meuer
Retinal Emboli and Stroke: The Beaver Dam Eye Study
Arch Ophthalmol, August 1, 1999; 117(8): 1063 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. E. Lee, B. E. K. Klein, and R. Klein
Changes in Refractive Error over a 5-Year Interval in the Beaver Dam Eye Study
Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1645 - 1649.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
D. Smith, F. Shang, T. R. Nowell, G. Asmundsson, G. Perrone, G. Dallal, L. Scott, M. Kelliher, B. Gindelsky, and A. Taylor
Decreasing Ascorbate Intake Does Not Affect the Levels of Glutathione, Tocopherol or Retinol in the Ascorbate-Requiring Osteogenic Disorder Shionogi Rats
J. Nutr., June 1, 1999; 129(6): 1229 - 1232.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
D. S. Hage
Affinity Chromatography: A Review of Clinical Applications
Clin. Chem., May 1, 1999; 45(5): 593 - 615.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Blincko and R. Edwards
Non-separation assay for glycohemoglobin
Clin. Chem., June 1, 1998; 44(6): 1302 - 1308.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
K. Ikeda, Y. Sakamoto, Y. Kawasaki, T. Miyake, K. Tanaka, T. Urata, Y. Katayama, S. Ueda, and S. Horiuchi
Determination of glycated albumin by enzyme-linked boronate immunoassay (ELBIA)
Clin. Chem., February 1, 1998; 44(2): 256 - 263.
[Abstract] [Full Text] [PDF]

Home page
 J CARDIOVASC PHARMACOL THERHome page
G.N. Jyothirmayi, B.J. Soni, M. Masurekar, M. Lyons, and T.J. Regan
Effects of Metformin on Collagen Glycation and Diastolic Dysfunction in Diabetic Myocardium
Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 1998; 3(4): 319 - 326.
[Abstract] [PDF]

Home page
 Clin. Chem.Home page
K. Miedema
Electrospray Mass Spectrometry for Measurement of Glycohemoglobin
Clin. Chem., May 1, 1997; 43(5): 705 - 707.
[Full Text] [PDF]

Home page
 CirculationHome page
A. Jain, G. Avendano, S. Dharamsey, A. Dasmahapatra, R. Agarwal, A. Reddi, and T. Regan
Left Ventricular Diastolic Function in Hypertension and Role of Plasma Glucose and Insulin : Comparison With Diabetic Heart
Circulation, April 1, 1996; 93(7): 1396 - 1402.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H. C. McGill Jr, C. A. McMahan, G. T. Malcom, M. C. Oalmann, and J. P. Strong
Relation of Glycohemoglobin and Adiposity to Atherosclerosis in Youth
Arterioscler. Thromb. Vasc. Biol., April 1, 1995; 15(4): 431 - 440.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the American Association for Clinical Chemistry.