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Clinical Chemistry 28: 2264-2268, 1982;
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Clinical Chemistry, Vol 28, 2264-2268, Copyright © 1982 by American Association for Clinical Chemistry

Liquid-chromatographic estimation of saturated phospholipid palmitate in amniotic fluid compared with a thin-layer chromatographic method for acetone-precipitated lecithin

SM Sax, JJ Moore, A Oley, JS Amenta and JA Silverman

The "high-performance" liquid-chromatographic method we report for measuring palmitate derived from saturated phospholipids in amniotic fluid is sensitive and precise and possesses a wide dynamic range. Palmitate from nonsurfactant sources is largely excluded. The procedure involves oxidation of the chloroform/methanol-extracted lipids with osmium tetroxide, precipitation of the resulting phosphatides with cold acetone, mild alkaline hydrolysis, derivatization of the liberated fatty acids to phenacyl esters, and reversed-phase chromatography. The method is no less convenient or more time-consuming than measurement of the lecithin/sphingomyelin ratio by thin-layer chromatography. The clinical usefulness of the procedure is indicated by results of a correlative study of 26 amniotic fluids evaluated by "lung profile" tests in a second laboratory. A value for dipalmitoyl lecithin of 13 mg/L or greater indicates fetal lung maturity.


The following articles in journals at HighWire Press have cited this article:


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 Obstet GynecolHome page
B. W. Fenton, C.-S. Lin, S. Ascher, and C. Macedonia
MAGNETIC RESONANCE SPECTROSCOPY TO DETECT LECITHIN IN AMNIOTIC FLUID AND FETAL LUNG
Obstet. Gynecol., March 1, 2000; 95(3): 457 - 460.
[Abstract] [Full Text] [PDF]


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