Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 28: 2294-2296, 1982;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakamura, J.
Right arrow Articles by Yakata, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakamura, J.
Right arrow Articles by Yakata, M.

Clinical Chemistry, Vol 28, 2294-2296, Copyright © 1982 by American Association for Clinical Chemistry

Urine contains an inhibitor for turbidimetric determinations of protein

J Nakamura and M Yakata

Our examination of urine components separated by gel filtration revealed the presence of an inhibitor that decreases the analytical recovery of protein in a turbidimetric assay involving sulfosalicylic acid as reagent (Proc. Soc. Exp. Biol. Med. 92: 748, 1956). The apparent relative molecular mass of this inhibitor was in the range 160 000-240 000. A study with purified proteins showed a similar inhibition by gamma-globulin, glycoprotein, and beta-lipoprotein in the assay of albumin by the same turbidimetric method. In contrast, measurement of protein by a dye binding method was not affected by these materials. The low values for apparent urinary protein given by the turbidimetric method as compared with those by the dye-binding method are at least partly ascribable to the inhibitor.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the American Association for Clinical Chemistry.