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Clinical Chemistry 28: 339-343, 1982;
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Clinical Chemistry, Vol 28, 339-343, Copyright © 1982 by American Association for Clinical Chemistry

Measurement of alpha-fetoprotein in maternal serum: three commercial radioimmunoassay kits and two non-commercial radioimmunoassays compared

JC Forest, F Verreault and M Pouliot

We evaluated three commercial radioimmunoassay kits (Amersham, Dainabot, Clinical Assays) and two non-commercial methods for determining alpha-fetoprotein in maternal serum during pregnancy. All five procedures were found to be acceptable with respect to practicability, sensitivity, linearity, and precision. Similar results were obtained with Dainabot, Clinical Assays, and the two non- commercial methods, but the Amersham method revealed a proportional error, results being about 20% lower than those by the other methods. Use of the international unit system is suggested for reporting results for AFP, to facilitate comparison between methods and laboratories.




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Copyright © 1982 by the American Association for Clinical Chemistry.