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Clinical Chemistry 28: 615-617, 1982;
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Clinical Chemistry, Vol 28, 615-617, Copyright © 1982 by American Association for Clinical Chemistry

Screening test for alpha 1-antitrypsin in dried-blood specimens

AP Orfanos, EW Naylor and R Guthrie

We describe a fluorescent spot test for detecting alpha 1-antitrypsin activity in dried-blood specimens. The eluate of a blood disc is mixed with carbobenzoxy-L-arginine-7-amino-4-methylcoumarin amide and trypsin at the appropriate pH. In the absence of alpha 1-antitrypsin, aminomethylcoumarin, a strongly fluorescent compound, is released. The reaction mixture, when spotted on chromatography paper and viewed under ultraviolet light, exhibits a bright fluorescence only in the case of specimens with alpha 1-antitrypsin deficiency. alpha 1-Antitrypsin activity so estimated correlated well with quantitative assays of dried- blood spots and serum. The procedure is simple and inexpensive, and has the potential for use as a screening test.




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Copyright © 1982 by the American Association for Clinical Chemistry.