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Clinical Chemistry 28: 819-823, 1982;
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Clinical Chemistry, Vol 28, 819-823, Copyright © 1982 by American Association for Clinical Chemistry

A normalized protein map of human plasma, and characterization of the normalized spots by microscale amino acid analysis

T Manabe, K Kojima, S Jitzukawa, T Hoshino and T Okuyama

A "normalized map" of human plasma proteins, which shows the standard distribution pattern of the proteins separated two-dimensionally in the absence of denaturing agents, is described. The pI and molecular mass of the protein represented by each spot in the normalized map are shown, and 84 of 128 spots are identified or tentatively identified as known plasma proteins. Of the several methods we used for the identification, the method of micro-scale amino acid analysis of the proteins extracted from the fixed, stained two-dimensional gels is described in detail. Patterns for human cerebrospinal fluid and urinary proteins are compared with the normalized map, the positions of proteins characteristic of each body fluid sample being shown.




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Copyright © 1982 by the American Association for Clinical Chemistry.