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Clinical Chemistry 28: 824-827, 1982;
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Clinical Chemistry, Vol 28, 824-827, Copyright © 1982 by American Association for Clinical Chemistry

Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine

T Manabe, E Hayama and T Okuyama

In this technique, in which no denaturing agent is used, proteins in human serum, cerebrospinal fluid, and urine are separated by isoelectric focusing in cylindrical 40 g/L polyacrylamide gels of capillary size (1.3 x 35 mm) for 40 min, followed by electrophoresis in 40--170 g/L polyacrylamide linear gradient gel, with use of 38 x 35 x 1 mm slab gel, for 1 h. Only 2 microL of untreated human serum is required to obtain clear protein-distribution patterns, made visible by Coomassie Blue staining. By use of silver staining, proteins in unconcentrated cerebrospinal fluid can be made visible. An apparatus we devised for microscale two-dimensional electrophoresis enables us to analyze eight protein samples simultaneously.


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 Biol. Reprod.Home page
R. Sasaki, T. Nakayama, and T. Kato
Microelectrophoretic Analysis of Changes in Protein Expression Patterns in Mouse Oocytes and Preimplantation Embryos
Biol Reprod, June 1, 1999; 60(6): 1410 - 1418.
[Abstract] [Full Text]


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Copyright © 1982 by the American Association for Clinical Chemistry.