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Clinical Chemistry 28: 1137-1139, 1982;
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Clinical Chemistry, Vol 28, 1137-1139, Copyright © 1982 by American Association for Clinical Chemistry

Liquid chromatography of codeine in plasma with fluorescence detection

IW Tsina, M Fass, JA Debban and SB Matin

This analytical method for easier determination of codeine in human plasma is based on "high-performance" liquid chromatography for separation and the natural fluorescence of codeine for detection. Codeine is extracted from alkalinized plasma with a mixture of hexane and dichloromethane, and the extract is further purified and chromatographed. The method can be used for routine assay of codeine at the concentrations of 10 micrograms/L or greater in human plasma. As little as 4 micrograms/L can be detected. Coefficients of variation for the assay of codeine in the concentration range of 10 to 100 micrograms/L were 2.2-7.4% (n = 6). We used this method to establish a concentration/time profile for plasma from a human volunteer after a 60- mg oral dose of codeine sulfate.




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Copyright © 1982 by the American Association for Clinical Chemistry.