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Clinical Chemistry 28: 1509-1513, 1982;
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Clinical Chemistry, Vol 28, 1509-1513, Copyright © 1982 by American Association for Clinical Chemistry

Radioimmunoassay for phencyclidine (PCP) in serum

SM Owens, J Woodworth and M Mayersohn

This accurate, sensitive radioimmunoassay for determining phencyclidine concentrations in serum specimens involves the use of anti- phencyclidine sera, 0.1 mL of serum specimen, an iodinated tracer, and a solid-phase separation. Phencyclidine metabolites do not show significant cross reactivity, but several phencyclidine analogs do cross react. Within-run coefficients of variation for human and dog serum ranged from 2.5 to 13% for concentrations from 2.0 to 500 micrograms/L. Day-to-day coefficients of variation for human and dog serum ranged from 4.3 to 16.7% for concentrations ranging from 2.0 to 90.0 micrograms/L. The sensitivity of the radioimmunoassay is less than 0.5 micrograms/L. Thirty serum specimens from two dogs given 1 mg of phencyclidine per kilogram body weight were analyzed by radioimmunoassay and a gas-chromatographic method. Nonparametric statistical comparison and linear regression showed that results from the two procedures correlate well (r2 = 0.952). Concentration-time data from the two dogs are presented to illustrate the utility of the radioimmunoassay for examining phencyclidine disposition.


The following articles in journals at HighWire Press have cited this article:


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 Drug Metab. Dispos.Home page
J. W. Proksch, W. B. Gentry, and S. M. Owens
The Effect of Rate of Drug Administration on the Extent and Time Course of Phencyclidine Distribution in Rat Brain, Testis, and Serum
Drug Metab. Dispos., July 1, 2000; 28(7): 742 - 747.
[Abstract] [Full Text]

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 J. Pharmacol. Exp. Ther.Home page
J. W. Proksch, W. B. Gentry, and S. M. Owens
Anti-Phencyclidine Monoclonal Antibodies Provide Long-Term Reductions in Brain Phencyclidine Concentrations during Chronic Phencyclidine Administration in Rats
J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 831 - 837.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. W. Proksch, W. B. Gentry, and S. M. Owens
Pharmacokinetic Mechanisms for Obtaining High Renal Coelimination of Phencyclidine and a Monoclonal Antiphencyclidine Antigen-Binding Fragment of Immunoglobulin G in the Rat
J. Pharmacol. Exp. Ther., November 1, 1998; 287(2): 616 - 624.
[Abstract] [Full Text]


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