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Clinical Chemistry 29: 1793-1795, 1983;
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Clinical Chemistry, Vol 29, 1793-1795, Copyright © 1983 by American Association for Clinical Chemistry

Determination of bethanidine in plasma by liquid-chromatography with a microbore reversed-phase column

JR Shipe, AF Arlinghaus, J Savory, MR Wills and JP DiMarco

In this novel method for quantifying bethanidine in plasma, after a multi-step extraction of bethanidine and internal standard from 2.0 mL of plasma, the drugs are separated on a "microbore" C18 reversed-phase column and quantified by their ultraviolet absorbance at 210 nm. The isocratic chromatographic separation takes about 15 min with use of an ion-pairing regent in the mobile phase (acetate buffer/acetonitrile, 9/1 by vol) and a flow rate of 0.25 mL/min. Sensitivity is increased relative to conventional columns, and solvent consumption is reduced by 90%. The standard curve is linear to at least 5 mg/L, and the detection limit is 0.02 mg/L. The within-run precision of the method is excellent (CV 4%) at a midrange concentration of 1.25 mg/L.




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Copyright © 1983 by the American Association for Clinical Chemistry.