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Clinical Chemistry 29: 862-867, 1983;
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Clinical Chemistry, Vol 29, 862-867, Copyright © 1983 by American Association for Clinical Chemistry

External quality assessment of assays for cortisol in plasma: use of target data obtained by gas chromatography/mass spectrometry

SJ Gaskell, CJ Collins, GC Thorne and GV Groom

We describe procedures for measuring cortisol in plasma and serum by isotope dilution and mass spectrometry. A method that incorporated solvent extraction, derivatization, and gas chromatography/high- resolution mass spectrometry provided data of good precision; interassay CVs were generally 3 to 4% for the concentration range of 100-650 nmol/L. Replacing solvent extraction with extraction on a column of Lipidex 1000 or extraction by immunoadsorption yielded data in excellent agreement with the first method. Plasma and serum pools were analyzed to provide target data for use in the U.K. National External Quality Assessment Scheme for cortisol assays. Routine laboratory assays, as judged by comparison with mass-spectrometric data, were generally positively biased except for analysis of a charcoal-stripped plasma supplemented with cortisol. The results emphasize the importance of using unadulterated plasma or serum pools in assessments of steroid assay procedures.


The following articles in journals at HighWire Press have cited this article:


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 Ann Clin BiochemHome page
L. Wood, D. H Ducroq, H. L Fraser, S. Gillingwater, C. Evans, A. J Pickett, D. W Rees, R. John, and A. Turkes
Measurement of urinary free cortisol by tandem mass spectrometry and comparison with results obtained by gas chromatography-mass spectrometry and two commercial immunoassays
Ann Clin Biochem, July 1, 2008; 45(4): 380 - 388.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Association for Clinical Chemistry.