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Clinical Chemistry 29: 1578-1581, 1983;
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Clinical Chemistry, Vol 29, 1578-1581, Copyright © 1983 by American Association for Clinical Chemistry

Simplified methods for measuring enzyme kinetics in a single cell

NS Markovic, OT Markovic and DS Young

We discuss techniques for measuring myeloperoxidase activity cytochemically in isolated polymorphonuclear neutrophils. We have used a sophisticated scanning microdensitometer as a reference device and compared results obtained by Zeiss Ultraphot III B and Reichert Zetopan microscopes with those of the scanning microdensitometer. We made both end-point and kinetic measurements, using conventional microscope slides and a special incubation chamber. Some resolution is lost with simpler microscope systems, but they provided results sufficiently like those obtained with the scanning microdensitometer that they could be used in the clinical laboratory to allow investigation of enzyme activity in single cells.




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Copyright © 1983 by the American Association for Clinical Chemistry.