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Clinical Chemistry 3: 178-184, 1957;
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Clinical Chemistry, Vol 3, 178-184, Copyright © 1957 by the American Association for Clinical Chemistry

Total Neutral 17-Ketosteroids

Clinical Method for Measurement

Marian S. Kafka 1 and Philip K. Bondy 1

1 Department of Internal Medicine, Yale University, New Haven, Conn.

The Masuda and Thuline method for measuring urinary neutral 17-ketosteroids has been modified by substituting polyethylene bottles and a mechanical shaker for separatory funnels and manual shaking, and by developing the color and reading it in the same test tube. Combining Zimmermann reagents before addition eliminates the necessity for frequently pipeting small volumes. The new method permits the analysis of 10 to 15 urines in a single operation without sacrificing accuracy or specificity.

Submitted on October 20, 1956




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Copyright © 1957 by the American Association for Clinical Chemistry.