Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 3: 185-194, 1957;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramsey, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramsey, H. A.

Clinical Chemistry, Vol 3, 185-194, Copyright © 1957 by the American Association for Clinical Chemistry

Photometric Procedure for Determining Esterase Activity

Harold A. Ramsey 1

1 Animal Nutrition Section, Department of Animal Industry, North Carolina State College, Raleigh, N. C.

A procedure for determining the esterase content of biologic tissues and fluids has been presented. Fatty acid esters of 2-naphthol are employed as substrates. The 2-naphthol liberated by hydrolysis is coupled with a diazonium salt to form an azo dye, the concentration of which is estimated photometrically. Distinctive features of this method are (1) the use of Brij 35 to prepare nonopalescent solutions of substrate,(2) the adjustment of pH to ensure complete formation of the azo dye, and (3) the solubilizing of the azo dye by adding sodium hydroxide. This assay procedure is sensitive to very small amounts of esterase, and the stability of its color is excellent if proper precautions are taken.

Submitted on December 8, 1956






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1957 by the American Association for Clinical Chemistry.