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Clinical Chemistry 32: 816-818, 1986;
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Clinical Chemistry, Vol 32, 816-818, Copyright © 1986 by American Association for Clinical Chemistry

Liquid-chromatographic determination of isoenzymes of alkaline phosphatase in serum and tissue homogenates

E Schoenau, KH Herzog and HJ Boehles

We describe a new method for separating alkaline phosphatase (AP) isoenzymes by means of "high-performance" liquid chromatography. Isoenzymes are eluted from the column (Mono Q HR 5/5, a strong anion- exchanger) with a stepwise gradient of LiCl. The isoenzymes originating from small intestine, bone, liver, and bile were identified by use of tissue homogenates, pathological sera, and heat inactivation.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
C. P. Price, T. P. Milligan, and C. Darte
Direct comparison of performance characteristics of two immunoassays for bone isoform of alkaline phosphatase in serum
Clin. Chem., November 1, 1997; 43(11): 2052 - 2057.
[Abstract] [Full Text] [PDF]


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Copyright © 1986 by the American Association for Clinical Chemistry.