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Clinical Chemistry 32: 1016-1019, 1986;
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Clinical Chemistry, Vol 32, 1016-1019, Copyright © 1986 by American Association for Clinical Chemistry

Simplified liquid-chromatographic determination of vancomycin

AF Rosenthal, I Sarfati and E A'Zary

We simplified determination of vancomycin in serum by using a direct and rapid solvent precipitation of protein and by using a simple organic compound as internal standard. Reproductibility of the vancomycin retention time was improved by modifying the mobile phase. Data obtained by fluorescence polarization immunoassay correlated well, and there was little bias between the two methods. The present method is sufficiently rapid to make this a practical choice for many laboratories.




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Copyright © 1986 by the American Association for Clinical Chemistry.