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Clinical Chemistry 32: 1473-1476, 1986;
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Clinical Chemistry, Vol 32, 1473-1476, Copyright © 1986 by American Association for Clinical Chemistry

Uniform chromatographic conditions for quantifying urinary catecholamines, metanephrines, vanillylmandelic acid, 5- hydroxyindoleacetic acid, by liquid chromatography, with electrochemical detection

NC Parker, CB Levtzow, PW Wright, LL Woodard and JF Chapman

Uniform liquid-chromatographic conditions were developed such that we could quantify norepinephrine, epinephrine, normetanephrine, metanephrine, vanillylmandelic acid, and 5-hydroxyindoleacetic acid in urine by using a single mobile phase of monochloroacetic acid and citric acid, 0.1 mol/L each. All compounds were separated on a C18 column and detected electrochemically at a potential of +0.800 V. Optimization of these uniform chromatographic conditions significantly shortens the changeover time required from one assay to another, resulting in a substantial savings of time and cost to the laboratory.


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