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Clinical Chemistry 32: 1631-1636, 1986;
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Clinical Chemistry, Vol 32, 1631-1636, Copyright © 1986 by American Association for Clinical Chemistry

Strand displacement applied to assays with nucleic acid probes

MS Ellwood, M Collins, EF Fritsch, JI Williams, SE Diamond and JG Brewen

This novel method for the detection of specific nucleic acid sequences has potential applications to clinical diagnosis. During hybridization, a signal-bearing nucleic acid strand is displaced by the target nucleic acid from a partially single-stranded complementary probe strand of nucleic acid. The probe:signal strand complex is prepared by hybridizing single-stranded probe that is entirely complementary to the target nucleic acid with a shorter signal sequence that is complementary to a portion of the probe strand. The sample nucleic acid is added to this hybrid complex under hybridization conditions. The target sequence, if present in the sample, will hybridize first to the unoccupied probe sequences, and then will displace the labeled strand by branch migration. By this "strand displacement" the signal strands are freed in solution, where they may be separated from those still hybridized; the quantity of label measured is directly proportional to the amount of analyte sequences in the sample. This method, demonstrated here for model and synthetic DNAs, can easily be adapted for the detection of any RNA or DNA sequence and obviates the need for immobilization of sample. A wide variety of labeling techniques can be used, and the displacement can be performed in solution or with the hybrid complex attached to a solid support. This assay circumvents nonspecific binding of label to the filter matrix and the laborious washing steps inherent in other assays involving nucleic acid probes.


The following articles in journals at HighWire Press have cited this article:


Home page
 Nucleic Acids ResHome page
Q. Li, G. Luan, Q. Guo, and J. Liang
A new class of homogeneous nucleic acid probes based on specific displacement hybridization
Nucleic Acids Res., January 15, 2002; 30(2): e5 - e5.
[Abstract] [Full Text] [PDF]

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 Appl. Environ. Microbiol.Home page
M. Mau and K. N. Timmis
Use of Subtractive Hybridization To Design Habitat-Based Oligonucleotide Probes for Investigation of Natural Bacterial Communities
Appl. Envir. Microbiol., January 1, 1998; 64(1): 185 - 191.
[Abstract] [Full Text]


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Copyright © 1986 by the American Association for Clinical Chemistry.