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Clinical Chemistry 32: 1742-1745, 1986;
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Clinical Chemistry, Vol 32, 1742-1745, Copyright © 1986 by American Association for Clinical Chemistry

A radioimmunoassay for the antimalarial drug chloroquine

C Freier, G Alberici, P Turk, F Baud and C Bohuon

We describe a 3H-based RIA for the antimalarial drug chloroquine (CLQ), the most commonly used antimalarial drug. In the assay a monoclonal antibody is used that is directed against 4-amino-7-chloroquinoline conjugated to keyhole limpet hemocyanin by the glutaraldehyde method. Besides CLQ, this antibody also recognizes with good affinity the 4- aminoquinoline homologs, hydroxychloroquine and amodiaquine. No extraction step or sample preparation is required, and the method can detect as little as 10 micrograms/L, the lower concentration in plasma of humans who are taking CLQ as a preventive measure. The between-assay CV is less than or equal to 10%, the within-assay CV less than or equal to 3%. Results correlate with those by liquid chromatography (r2 = 0.96). The speed and simplicity of the RIA method make it useful in evaluating the CLQ concentrations in acutely toxic patients.




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Copyright © 1986 by the American Association for Clinical Chemistry.