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Clinical Chemistry 34: 106-109, 1988;
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Clinical Chemistry, Vol 34, 106-109, Copyright © 1988 by American Association for Clinical Chemistry

Simple, rapid enzymatic determination of glycerophosphocholine in human seminal plasma

HJ Chap, JP Moatti, R Mieusset, M Nieto, G Laneelle, PJ Bennet, A Mansat, F Pontonnier and L Douste-Blazy
Laboratoire de Biochimie III, Hopital La Grave, Toulouse, France.

We have devised a new enzymatic determination of sn-glycero-3- phosphocholine (GPC) in human seminal plasma. This is based on GPC hydrolysis by a phosphodiesterase (PDE), free choline being then determined by the choline oxidase method. The whole procedure involves a first incubation in the presence of choline oxidase and catalase, to eliminate the excess of choline present in seminal plasma (10-fold, compared with GPC). Absorbance and concentration are linearly related up to at least 100 nmol per assay, analytical recovery ranges between 89% and 105%, and intra- and interassay CVs are 3.2% and 5.6%, respectively, at the highest substrate concentration. Using this procedure, we found seminal plasma from 21 fertile men to contain 5.22 (SD 3.33) mumol per ejaculate--within the same range as previously reported values obtained chromatographically. After vasectomy, GPC in seminal plasma decreased to 28% of its original value, as determined in 10 volunteers. Thus this new method displays appropriate characteristics of specificity, reliability, and convenience, allowing its use in routine evaluation of male fertility.


The following articles in journals at HighWire Press have cited this article:


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 J. Biol. Chem.Home page
C. Delagebeaudeuf, A. Gassama-Diagne, M. Nauze, A. Ragab, R. Y. Li, J. Capdevielle, P. Ferrara, J. Fauvel, and H. Chap
Ectopic Epididymal Expression of Guinea Pig Intestinal Phospholipase B. POSSIBLE ROLE IN SPERM MATURATION AND ACTIVATION BY LIMITED PROTEOLYTIC DIGESTION
J. Biol. Chem., May 29, 1998; 273(22): 13407 - 13414.
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Copyright © 1988 by the American Association for Clinical Chemistry.