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Clinical Chemistry 34: 163-164, 1988;
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Clinical Chemistry, Vol 34, 163-164, Copyright © 1988 by American Association for Clinical Chemistry

Isolation of the acidic phospholipid phosphatidylglycerol from pulmonary surfactant by sorbent extraction chromatography

J Egberts and R Bulskool
Department of Obstetrics & Gynecology, Leiden University Medical Center, The Netherlands.

Aminopropyl solid-phase columns can be used for phospholipid fractionation. A method has been developed to elute the acidic phospholipid phosphatidylglycerol (PG) separately, and it is applicable to both standard (phospho)lipid mixtures and pulmonary surfactants. The simplicity, rapidity, and high recovery make this method of isolating PG superior to other chromatographic procedures.





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Copyright © 1988 by the American Association for Clinical Chemistry.