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Clinical Chemistry 34: 165, 1988;
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Clinical Chemistry, Vol 34, 165-165, Copyright © 1988 by American Association for Clinical Chemistry

Chemical assay of p-aminohippuric acid simplified by use of dimethylaminocinnamaldehyde in ethanol

LB Schwartz, BL Gewertz and MG Bissell
University of Chicago Pritaker School of Medicine, IL.

We have developed a new and simple method of p-aminohippuric acid determination by use of dimethylaminocinnamaldehyde (DACA). It differs from previous methods using DACA in that the reaction is carried out in ethanol rather than in dilute acid. This results in deeper and more stable color development. We have used this method successfully to determine effective renal plasma flow in a clinical study of this variable in pregnant women.


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Copyright © 1988 by the American Association for Clinical Chemistry.