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Clinical Chemistry 34: 2066-2069, 1988;
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Clinical Chemistry, Vol 34, 2066-2069, Copyright © 1988 by American Association for Clinical Chemistry

Estradiol-17 beta quantified in serum by isotope dilution-gas chromatography-mass spectrometry: reversed-phase C18 high-performance liquid chromatography compared with immuno-affinity chromatography for sample pretreatment

LM Thienpont, PG Verhaeghe, KA Van Brussel and AP De Leenheer
Laboratoria voor Medische Biochemie en voor Klinische Analyse, Rijksuniversiteit Gent, Belgium.

Here, isotope dilution-gas chromatography-mass spectometry is used as a reference technique for determining the concentration of estradiol-17 beta in candidate human serum Reference Material. The accuracy of assigned concentrations in biologic materials is not only determined by instrumental performance, it also depends greatly on the selectivity of the procedure for isolating the analyte from the biological matrix, an issue which we consider insufficiently addressed in the literature. We introduced reversed-phase C18 high-performance liquid chromatography as a fractionation procedure in addition to the commonly used solvent extraction and column chromatography on Sephadex LH-20. The validity of this approach as part of a Reference Method for measurement of estradiol-17 beta by isotope dilution-gas chromatography-mass spectrometry was investigated by comparison with immuno-affinity chromatography, which on theoretical grounds is generally considered as highly selective.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
Z. Cao, T. A. Swift, C. A. West, T. G. Rosano, and R. Rej
Immunoassay of Estradiol: Unanticipated Suppression by Unconjugated Estriol
Clin. Chem., January 1, 2004; 50(1): 160 - 165.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
H. Wu, C. Ramsay, P. Ozaeta, L. Liu, and H. Aboleneen
Serum Estradiol Quantified by Isotope Dilution-Gas Chromatography/Mass Spectrometry
Clin. Chem., February 1, 2002; 48(2): 364 - 366.
[Full Text] [PDF]


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