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Clinical Chemistry 34: 2291-2294, 1988;
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Clinical Chemistry, Vol 34, 2291-2294, Copyright © 1988 by American Association for Clinical Chemistry

New enzymatic determination of sialic acid in serum

S Teshima, K Tamai, Y Hayashi and S Emi
Tsuruga Enzyme Plant, Toyobo Co. Ltd., Fukui, Japan.

This enzymatic method for determination of sialic acid involves use of neuraminidase (EC 3.2.1.18), N-acetylneuraminate lyase (EC 4.1.3.3), acylglucosamine 2-epimerase (EC 5.1.3.8), N-acetylhexosamine oxidase (from Pseudomonas sp.), and peroxidase (EC 1.11.1.7). Because the method does not require pyruvic acid in the assay medium, interference by pyruvic acid in serum can be avoided. This simple, accurate assay is little affected by other substances in serum.




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Copyright © 1988 by the American Association for Clinical Chemistry.