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Clinical Chemistry 34: 2308-2310, 1988;
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Clinical Chemistry, Vol 34, 2308-2310, Copyright © 1988 by American Association for Clinical Chemistry

A re-examination of the stability of retinol in blood and serum, and effects of a standardized meal

MH Barreto-Lins, FA Campos, MC Azevedo and H Flores
Departamento de Nutricao, Universidade Federal de Pernambuco, Recife, Brasil.

We examined the stability of retinol in blood and serum samples, kept in the dark, under different handling procedures. Samples not protected from contact with air oxygen were highly unstable, even when kept at ice temperature. Samples collected under anaerobic conditions, with Vacutainer Tubes, or treated with nitrogen after collection to displace the air from the tubes were stable during the usual interval between collection and freezing or analysis in biochemical surveys. Ingestion of a moderate amount of vitamin. A significantly increased serum retinol concentrations in normal volunteers, showing the importance that survey samples be preprandial.


The following articles in journals at HighWire Press have cited this article:


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M. Fujita, E. Brindle, J. Shofer, P. Ndemwa, Y. Kombe, B. Shell-Duncan, and K. A. O'Connor
Retinol-Binding Protein Stability in Dried Blood Spots
Clin. Chem., November 1, 2007; 53(11): 1972 - 1975.
[Abstract] [Full Text] [PDF]

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M. van Eijsden, M. F. van der Wal, G. Hornstra, and G. J. Bonsel
Can Whole-Blood Samples Be Stored over 24 Hours without Compromising Stability of C-Reactive Protein, Retinol, Ferritin, Folic Acid, and Fatty Acids in Epidemiologic Research?
Clin. Chem., January 1, 2005; 51(1): 230 - 232.
[Full Text] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.