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Clinical Chemistry 34: 2320-2322, 1988;
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Clinical Chemistry, Vol 34, 2320-2322, Copyright © 1988 by American Association for Clinical Chemistry

Homogeneous time-resolved fluoroimmunoassay of thyroxin in serum

I Hemmila, O Malminen, H Mikola and T Lovgren
Wallac Chemical Laboratories, Turku, Finland.

We describe a rapid, simple nonseparation fluoroimmunoassay for determination of thyroxin in serum. The assay is based on the labeling of thyroxin directly with a fluorescent europium chelate, the fluorescence of which is quenched on binding to an antithyroxin antibody. With the assay buffer we used, maximum quenching is 90%. The rapid achievement of equilibrium in the assay solution, regardless of the sequence of reagent additions, allows fast measurement of thyroxin. Precision was good (CV less than 5%) within the clinical range for total thyroxin (50-300 nmol/L), and results correlated well with those by a commercial radioimmunoassay.


The following articles in journals at HighWire Press have cited this article:


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 J Biomol ScreenHome page
I. Hemmila
LANCETM: Homogeneous Assay Platform for HTS
J Biomol Screen, December 1, 1999; 4(6): 303 - 307.
[PDF]

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 Clin. Chem.Home page
K. Blomberg, P. Hurskainen, and I. Hemmila
Terbium and Rhodamine as Labels in a Homogeneous Time-resolved Fluorometric Energy Transfer Assay of the ß Subunit of Human Chorionic Gonadotropin in Serum
Clin. Chem., June 1, 1999; 45(6): 855 - 861.
[Abstract] [Full Text] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.