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Clinical Chemistry 34: 2406-2409, 1988;
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Clinical Chemistry, Vol 34, 2406-2409, Copyright © 1988 by American Association for Clinical Chemistry

Alkaline phosphatase isoenzymes in serum determined by high-performance anion-exchange liquid chromatography with detection by enzyme reaction

MT Parviainen, JH Galloway, JH Towers and JA Kanis
Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.

This rapid, reproducible method for separating and determining individual alkaline phosphatase (EC 3.1.3.1) isoenzymes in serum is based on high-performance liquid chromatography with a weak anion- exchange column (SynChropak AX 300). The isoenzymes so resolved are detected by using an on-line enzyme reaction followed by spectrophotometric monitoring at 405 nm of the 4-nitrophenol formed. Complete diagnostic profiles of the various isoenzymes present in normal and pathological sera are obtained within 20 min. The mean (and SD) normal concentrations of the bone B1 and intestinal isoenzymes in serum of adults were 3.7 (4.3) and 4.5 (3.9) U/L, respectively (n = 14), and of the bone isoenzyme B2 and liver isoenzymes L1 and L2, 5.8 (8.6), 33.0 (10.6), and 12.0 (4.8) U/L, respectively (n = 17). Concentrations of the B2 and L1 isoenzymes in adults over age 40 years differed significantly from those in adults younger than 40 years, that of bone isoenzyme being lower (P less than 0.05) and that of the liver isoenzyme being higher (P less than 0.001) in the younger adults.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
C. P. Price, T. P. Milligan, and C. Darte
Direct comparison of performance characteristics of two immunoassays for bone isoform of alkaline phosphatase in serum
Clin. Chem., November 1, 1997; 43(11): 2052 - 2057.
[Abstract] [Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
A.-M. Heikkinen, M. Parviainen, L. Niskanen, M. Komulainen, M. T. Tuppurainen, H. Kroger, and S. Saarikoski
Biochemical Bone Markers and Bone Mineral Density during Postmenopausal Hormone Replacement Therapy with and without Vitamin D3: A Prospective, Controlled, Randomized Study
J. Clin. Endocrinol. Metab., August 1, 1997; 82(8): 2476 - 2482.
[Abstract] [Full Text] [PDF]


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