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Clinical Chemistry 34: 2502-2503, 1988;
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Clinical Chemistry, Vol 34, 2502-2503, Copyright © 1988 by American Association for Clinical Chemistry

Liquid-chromatographic quantification compared with gas-chromatographic- mass-spectrometric determination of verapamil and norverapamil in plasma

PA Hynning, P Anderson, U Bondesson and LO Boreus
Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden.

A high-performance liquid chromatographic (HPLC) method for determining verapamil and norverapamil in plasma is presented and compared with gas chromatography/mass spectrometry (GC-MS). The plasma samples were extracted at alkaline pH with hexane containing 2-butanol (20 mL/L) and then back-extracted into phosphate buffer (0.1 mol/L, pH 3.0). For chromatography we used a reversed-phase column (Supelcosil LC-18 DB) with a mobile phase of the phosphate buffer and acetonitrile (70/30 by vol). Fluorescence detection was used (excitation at 203 nm, emission at 320 nm). Overall analytical recovery was 85%. Standard curves were linear from 1 to 1000 micrograms/L. The detection limit was 1 microgram/L. The assays are accurate and precise. We found no interferences by those substances tested. Results by HPLC and GC-MS agreed well (r = 0.99) for both verapamil and norverapamil determinations.


The following articles in journals at HighWire Press have cited this article:


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 Hum Exp ToxicolHome page
A Tracqui, C Tournoud, P Kintz, M Villain, C Kummerlen, P Sauder, and B Ludes
HPLC/MS findings in a fatality involving sustained-release verapamil
Human and Experimental Toxicology, September 1, 2003; 22(9): 515 - 521.
[Abstract] [PDF]

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 Hum Exp ToxicolHome page
K. T Kivistö, P. J Neuvonen, and L. Tarssanen
Pharmacokinetics of verapamil in overdose
Human and Experimental Toxicology, January 1, 1997; 16(1): 35 - 37.
[Abstract] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.