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Clinical Chemistry, Vol 34, 2510-2513, Copyright © 1988 by American Association for Clinical Chemistry
HM van Eijk, MA van der Heijden, CL van Berlo and PB Soeters
University Hospital Maastricht, Department of Surgery, The Netherlands.
This inexpensive method for fully automated amino acid analysis combines the advantages of automated precolumn derivatization with o- phthaldialdehyde and favorable analytical conditions to separate and quantify 30 amino acids found in normal plasma. The system can run unattended for almost four days, during which the data are processed automatically by a personal computer and a maximum of 76 samples and 19 standards can be processed (cycle time per analysis: 55 min). Only 1 microL of deproteinized plasma is required per analysis. Coefficients of variation for retention times and areas measured for all relevant amino acids are less than 1% and 3%, respectively. The system described is well suited for quick, sensitive operation in daily practice.
The following articles in journals at HighWire Press have cited this article:
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  M. P. Engelen, E. P. Rutten, C. L. De Castro, E. F. Wouters, A. M. Schols, and N. E. Deutz Supplementation of soy protein with branched-chain amino acids alters protein metabolism in healthy elderly and even more in patients with chronic obstructive pulmonary disease Am. J. Clinical Nutrition, February 1, 2007; 85(2): 431 - 439. [Abstract] [Full Text] [PDF]
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 E. P. A. Rutten, M. P.K.J. Engelen, C. L. N. Castro, E. F. M. Wouters, A. M.W.J. Schols, and N. E. P. Deutz Decreased Whole-Body and Splanchnic Glutamate Metabolism in Healthy Elderly Men and Patients with Chronic Obstructive Pulmonary Disease in the Postabsorptive State and in Response to Feeding J. Nutr., September 1, 2005; 135(9): 2166 - 2170. [Abstract] [Full Text] [PDF]
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 M.P.K.J. Engelen, M. Orozco-Levi, N.E.P. Deutz, E. Barreiro, N. Hernandez, E.F.M. Wouters, J. Gea, and A.M.W.J. Schols Glutathione and glutamate levels in the diaphragm of patients with chronic obstructive pulmonary disease Eur. Respir. J., April 1, 2004; 23(4): 545 - 551. [Abstract] [Full Text] [PDF]
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M. P. Engelen, E. F. Wouters, N. E. Deutz, P. P. Menheere, and A. M. Schols Factors contributing to alterations in skeletal muscle and plasma amino acid profiles in patients with chronic obstructive pulmonary disease Am. J. Clinical Nutrition, December 1, 2000; 72(6): 1480 - 1487. [Abstract] [Full Text] [PDF]
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 B. J.-W. Van Klinken, A. W. C. Einerhand, L. A. Duits, M. K. Makkink, K. M. A. J. Tytgat, I. B. Renes, M. Verburg, H. A. Buller, and J. Dekker Gastrointestinal expression and partial cDNA cloning of murine Muc2 Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G115 - G124. [Abstract] [Full Text] [PDF]
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 J. Le Boucher, C. Charret, C. Coudray-Lucas, J. Giboudeau, and L. Cynober Amino acid determination in biological fluids by automated ion-exchange chromatography: performance of Hitachi L-8500A Clin. Chem., August 1, 1997; 43(8): 1421 - 1428. [Abstract] [Full Text] [PDF]
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M. S. Rashed, M. P. Bucknall, D. Little, A. Awad, M. Jacob, M. Alamoudi, M. Alwattar, and P. T. Ozand Screening blood spots for inborn errors of metabolism by electrospray tandem mass spectrometry with a microplate batch process and a computer algorithm for automated flagging of abnormal profiles Clin. Chem., July 1, 1997; 43(7): 1129 - 1141. [Abstract] [Full Text] [PDF]
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